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JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ). The accumulation of JC-1 in these organelles leads to the formation J-ag …

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JC1 - Mitochondrial Membrane Potential Assay Kit (ab113850). HL60 cells were seeded and labeled according to section 11.1 of the protocol. Cells were then treated for 4 hours with a titration series of the thiazolidinedione Troglitazone and both monomer and aggregate forms were read on a Perkin Elmer-Wallac 1420 Victor 2 Multilabel plate reader.

Jul 7, 2017. I try to measure changes in mitochondrial membrane potential in A549 cells by FACS with the following protocol: -Dilution of stock solution of JC-1 (2.5mg/ml in DMSO) in HBSS with Ca ...

1. JC-11× Incubation Buffer 5 ； 2. 900µL JC-1 100µL 10-100 ug,37℃,5%CO 2 10~20min； 3. 、。 A.

Cayman's JC-1 Mitochondrial Membrane Potential Assay Kit provides all the necessary reagents, as well as complete instructions, for analysis of mitochondrial integrity in whole cells. The assay is applicable to fluorescence microscopy and fluorescent plate reader. For flow cytometry, Cayman's JC-1 Mitochondrial Membrane Potential Flow ...

1.JC-1：. 1.1DMSO210 mM JC-1。. ; <-20℃。. ：,。. 1.21X JC-1：,JC-1DMSOJC-1 …

JC-1CBIC2 (3), (mitochondrial membrane potential) Ψm,C25H27Cl4IN4。 JC-1 JC-1 C25H27Cl4N4.I 652.2251 CAS 1 2 JC-1CBIC2 (3)5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide,C 25 H 27 Cl 4 IN 4, …

JC-1 dye is a sensitive marker for mitochondrial membrane potential change. Generally, it gives Red fluorescence, but in case of mitochondrial membrane potential change, the dye gives green ...

JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis. 1,2,3 It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm).In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which …

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5492929. Scientific Background. JC-1 is a fluorescent dye that is able to pass across the mitochondrial membrane. It is used to measure mitochondrial membrane potential. At low membrane potentials, it remains a monomer and emits green-fluorescent light (λex=485 nm, λem=520 nm). At higher membrane potentials, it forms "J-aggregates", which ...

Key features and details. Mitochondrial membrane-potential dye. CAS Number: . Purity: > 98%. Soluble in DMSO to 25 mM. Form / State: Solid.

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1(3) 5 mg † * JC-1 concentrations of 1–5 mg/mL correspond to 1.5–7.7 mM, MW = 652. † JC-9 concentrations of 1–5 mg/mL correspond to 1.9–9.4 mM, MW = 532. Approximate fluorescence excitation/emission maxima: 514/529 nm, monomer form; 585/590 nm J-aggregate form. JC-1 and JC-9 Mitochondrial Potential Sensors Introduction

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JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. In this study, we show that in practice this …

9th Edition, revised in January, 2021 2 Elabscience®（JC-1）JC-1, 。 （CCCP）[E-CK-A301C]

JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial ...

. JC-1 MitoMP Detection Kitは、ミトコンドリアをできるキットです。. 、JC-1はの、けづらいのがありますが、キットはによりそれをしております。. さらに、キットにまれるImaging Bufferをいる ...

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